DETEKSI GEN SLC30A8 PADA SAMPEL DARAH PENDERITA DIABETES MELITUS TIPE 2 DENGAN METODE POLYMERASE CHAIN REACTION

Dinda, Helsa Auliantika (2023) DETEKSI GEN SLC30A8 PADA SAMPEL DARAH PENDERITA DIABETES MELITUS TIPE 2 DENGAN METODE POLYMERASE CHAIN REACTION. Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

The SLC30A8 gene is a zinc efflux transporter associated with insulin secretion expressed by the pancreas. The SLC30A8 gene causes abnormalities in insulin synthesis, maturation & secretion as well as abnormalities in the body's effectiveness in responding to glucose metabolism. Polymerase Chain Reaction (PCR) is a DNA multiplication method that can be used to detect the presence of the SLC30A8 gene in type 2 diabetes mellitus patients. The purpose of this study was to determine whether or not the SLC30A8 gene was present in blood samples of type 2 diabetes mellitus patients using the PCR method. This study used a descriptive research method conducted at the Molecular Biology Laboratory of Sekolah Tinggi Ilmu Kesehatan Nasional. The sampling technique in this study used a total sampling technique on 25 type 2 diabetes mellitus patients who were members of the Prolanis Diabetes Mellitus Puskesmas Bulu Sukoharjo. The detection of the SLC30A8 gene went through several stages, namely DNA isolation, qualitative testing of DNA isolates, quantitative testing of DNA isolates and PCR. The results showed the presence of the SLC30A8 gene in all blood samples from type 2 diabetes mellitus patients using the PCR method. The primer used with forward primer 5'-GGACGAAAGAGTTCCCATAGCG-3' and reverse primer 5'- ATAGCAGCATGTTTTGAAGGTGGC-3' attaches specifically to chromosome 8 with a product length of 429 bp. The SLC30A8 gene can be detected using the PCR method in blood samples of type 2 diabetes mellitus patients who are members of prolanis diabetes mellitus at Puskesmas Bulu Sukoharjo with a percentage of 100%.

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