PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK BUAH GAMBAS (Luffa acutangula(L.) Roxb.) DENGAN METODE SPEKTROFOTOMETRI UV-VIS

Tutik, Nur Hayati (2020) PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK BUAH GAMBAS (Luffa acutangula(L.) Roxb.) DENGAN METODE SPEKTROFOTOMETRI UV-VIS. Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Gambas or oyong fruit has various benefits, namely enlargement of lymph glands, diuretics, laksativa, antioxidants, hepatoprotective, inhibition of fungi and bacteria. This study aims to determine the level of total flavonoid compounds luffa fruit (Luffa acutangula (L.) Roxb). The extraction method uses socletation with 96% ethanol solvent. The extract obtained was used for qualitative and quantitative analysis. Phytochemical analysis of flavonoid content in samples using the Shinoda test, 10% NaOH solution, and concentrated H2SO4 solution, obtained positive results containing flavonoids. Quantitative analysis uses UV-Vis spectrophotometry at a maximum wavelength of 429.5 nm and an operating time of 30 minutes. The average total flavonoid level obtained was 9,897 ± 0.11 mg / gram extract with% KV 1.14%.

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