PENETAPAN KADAR LIKOPEN DALAM JUS JAMBU BIJI MERAH (Psidium guajava L.) DENGAN METODE SPEKTROFOTOMETRI UV – Vis

Aprilia, Asri Hastuti (2021) PENETAPAN KADAR LIKOPEN DALAM JUS JAMBU BIJI MERAH (Psidium guajava L.) DENGAN METODE SPEKTROFOTOMETRI UV – Vis. Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Red Guava Fruit (Psidium guajava L.) is a fruit that rich in phytochemicals that are beneficial to health. Lycopene is a carotenoid class compound that has antioxidant potential and is linked to the immune system, which plays an important role in disease prevention by maintaining sufficient function of immune cells to deal with homeostatics caused by oxidative stress. The purpose of this study was to determine the levels of lycopene in red guava (Psidium guajava L.) juice using the UV-Vis spectrophotometric method. The juice of the red guava fruit was extracted into a thick extract, then a qualitative test was carried out using the UV-Vis and TLC spectrophotometric methods and the concentration was carried out using the Lambert-Beer equation of the lycopene absorbance of the test solution measured by UV-Vis Spectrophotometry at a wavelength of 470 nm. The results of this study showed that red guava juice (Psidium guajava L.) was positive for lycopene with an average level of 2.9096 mg/100 gram of juice, with a %KV value of 15,8866.

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