PEMANFAATAN SOFTWARE ImageJ SEBAGAI ALTERNATIF DALAM MENENTUKAN DERAJAT PARASITEMIA MALARIA

Kurniawan, Try Susilo (2022) PEMANFAATAN SOFTWARE ImageJ SEBAGAI ALTERNATIF DALAM MENENTUKAN DERAJAT PARASITEMIA MALARIA. Other thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Malaria is an infectious disease caused by Plasmodium parasites that grow and multiply in human blood cells. The amount of Plasmodium in the blood can be determined by calculating the degree of parasitemia. The degree of parasitemia is the percentage of individuals in the population whose blood smears show parasites by examining both thick and thin blood smears. Parasite density can be seen in two ways, namely qualitative and semi-quantitative. The World Health Organization (WHO) requires that the diagnosis of malaria must be established as well as possible, one of which is by well-blood smear staining. The diagnosis of malaria infection is carried out by observing the parasite in the preparation or blood smear under a microscope after being stained with Giemsa at a certain concentration. ImageJ is one of digital image processing program that is used to calculate and analyze images by using a computer. The purpose of this research is to provide information about the use of ImageJ software in determining the degree of malaria parasitemia. The population of this research was Plasmodium from all malaria-positive stored preparations. Sampling technique is by means of Total Sampling. The thin blood preparations stain by Giemsa Method Staining. The slide was rinsed with running water slowly and then it was dried. Malaria preparations were observed and counted microscopically with 1000x magnification. The results obtained from manual calculations will be used as controls. Observations and calculations were continued quantitatively, starting with documenting images of the microscope's field of view using a digital camera. After obtaining the image, digital image processing was carried out by using the ImageJ program and the speed and accuracy were measured during the malaria calculation. This study uses a Analytic experimental design with T-Test Paired Method. The results obtained indicate that the calculation of the degree of parasitemia using ImageJ has a fairly high accuracy value of 86% and significany 0,527. The length of time the calculation process with Image J software is obtained is faster on average 06 minutes per slide. In summary the conclusion is that Image J software can be used as an alternative in determining the degree of malaria parasitemia.

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