PENGARUH PENAMBAHAN OKSIDATOR PADA AIR RENDAMAN ANGKAK DAN DAUN JATI TERHADAP HASIL MODIFIKASI PEWARNAAN GRAM

Nurhidayat, Nurhidayat (2022) PENGARUH PENAMBAHAN OKSIDATOR PADA AIR RENDAMAN ANGKAK DAN DAUN JATI TERHADAP HASIL MODIFIKASI PEWARNAAN GRAM. Other thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

The use of safranin has a weakness, namely the price is expensive and the color is difficult to absorb in certain preparations. The use of natural dyes is an alternative to replace safranin dyes. One of the natural dyes that can be used is water immersion Angkak and teak leaves. The addition of an oxidizing agent to natural dyes resulted in a clean field of view and a perfect bacterial shape. The purpose of this study was to determine the effect of adding an oxidizing agent to the immersion water of angkak and teak leaves on the modification of Gram staining. The research design was descriptive with an experimental approach to see the effect of adding oxidizing agents KMnO₄ and H₂O₂ to natural dyes soaked in Angkak and teak leaves on gram-positive and gram-negative bacteria by observing the parameters of visual field clarity, color contrast and the perfection of the shape of the stained bacteria. Sampling technique using probability sampling method with simple random sampling. The results showed that the staining of Staphylococcus aureus bacteria gave good results and the morphology could be observed and identified, while the Escherichia.coli bacteria were only stained with the cells, while the morphology could not be identified. The conclusion of this study was that there was no effect of adding an oxidizing agent to the immersion water of angkak and teak leaves on the modified Gram staining results.

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