ANALISIS PENGHAMBATAN KOLAGEN DARI KULIT DAN TULANG BELUT SAWAH TERHADAP REAKSI OKSIDASI DARI RADIKAL BEBAS DENGAN METODE DPPH DAN FRAP

Feransiska, Marentina Widyasari (2020) ANALISIS PENGHAMBATAN KOLAGEN DARI KULIT DAN TULANG BELUT SAWAH TERHADAP REAKSI OKSIDASI DARI RADIKAL BEBAS DENGAN METODE DPPH DAN FRAP. Other thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

The skin and bones of asian swamp eel (Monopterus albus) contain collagen compounds which can be used as antioxidants. This study aims to isolate collagen from the skin and bones of asian swamp eel and to determine the antioxidant activity and to determine the antioxidant activity using DPPH and FRAP method which are significantly different or not in measuring antioxidant activity. The isolation of the skin and bones of asian swamp eel with pretreatment using the base NaOH, and hydrolysis using acetic acid (CH3COOH), and salting out using NaCl. The obtained isolates were analyzed for their antioxidant activity using the DPPH and FRAP methods. The results of testing for antioxidant activity using the DPPH method obtained average IC50 values of 24,28±0,42 ppm. Tests using the FRAP method obtained average IC50 values of 20,83±0,24 ppm. Statistical analysis using the One sample T-test showed that there was a significant difference (p <0.05) between the DPPH and FRAP methods.

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