GAMBARAN MAKROSKOPIS JARINGAN HEPAR TIKUS (Rattus norvegicus) YANG DIPROSES MENGGUNAKAN MINYAK CENGKEH (Syzigium aromaticum) SEBAGAI ALTERNATIF LARUTAN CLEARING

Yulita, Maulani (2020) GAMBARAN MAKROSKOPIS JARINGAN HEPAR TIKUS (Rattus norvegicus) YANG DIPROSES MENGGUNAKAN MINYAK CENGKEH (Syzigium aromaticum) SEBAGAI ALTERNATIF LARUTAN CLEARING. Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

The best tissue preparations that are able to describe the condition of cells or tissues as they were still in the body. In the process of making histological tissue preparations, xylol is a solution that is often used in routine histology laboratories in the clearing process, but xylol is a flammable and colorless liquid, dissolved with some organic solvents and paraffin wax. Therefore, clove oil is used as a substitute for xylol. Clove oil is a class of hydrocarbon compounds and contains of essential oils. Clove oil has similar properties to xylol. It is safer, non-flammable and minimal in toxicity.The purpose of this study was to determine the potential of clove oil as an alternative agent for the clearing process.This research is experimental and divided into two groups namely xylol and clove oil. Each group amounted to 16 preparations. Rat liver tissue preparations were observed macroscopically.The results of measuring the quality of preparations descriptively showed the same results in both groups. The results of differences in xylol groups and clove oil which were statistically tested by the Mann Whitney test on each parameter showed a value of P <0.05. From these results, it can be concluded that clove oil can be used as an alternative medium to replace xylol solution.

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