IDENTIFIKASI β-GLUKAN JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq.) Kummer) DENGAN METODE FOURIER-TRANSFORM INFRARED SPECTROSCOPY (FTIR)

Christa, Marthaningsih Prisida (2020) IDENTIFIKASI β-GLUKAN JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq.) Kummer) DENGAN METODE FOURIER-TRANSFORM INFRARED SPECTROSCOPY (FTIR). Other thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

The white oyster mushroom (Pleurotus ostreatus (Jacq.) Kummer) βglucan-containing compounds that have potential activity as antitumor, antioxidant, anticholesterol, antiaging, and immune enhancer. A research has been done on the white oyster mushroom (Pleurotus ostreatus (Jacq.) Kummer) through determination to identify β-glucan contained in the white oyster mushroom (Pleurotus ostreatus (Jacq.) Kummer). Isolation process was conducted by Yap and Ng method based on the principle of boiling with distilled water by 100ºC followed by ethanol 96% precipitation by 4ºC and then drying by freeze drying. Characterization of the extracts through by Fourier-transform Infrared Spectroscopy (FTIR). The result showed that the extraction of 3 kilograms white oyster mushroom (Pleurotus ostreatus (Jacq.) Kummer) with Yap and Ng method with modification produced total yields as much as 0,00013%. FTIR characterization of molecular bonds identified in β-glucan are O-H which is characterized by the presence of an absorption peak at a wavelength of 3418,97 cm-1 , C-H which is characterized by the presence of an absorption peak at a wavelength of 2928,07 cm-1 , C-O-C which is characterized by the presence of an absorption peak at a wavelength of 1242,21 cm-1 , C-O which is characterized by the presence of an absorption peak at a wavelength of 1043,53-1076,33 cm-1 , and β-(1,3)-D-glukan which is characterized by the presence of an absorption peak at a wavelength of 1076,33 cm-1

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