PENETAPAN KADAR FLAVONOID TOTAL DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAN FRAKSI DAUN KLUWIH (Artocarpus camansi) DENGAN METODE ABTS

Yachinta, Aprilliani Kusuma W (2020) PENETAPAN KADAR FLAVONOID TOTAL DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAN FRAKSI DAUN KLUWIH (Artocarpus camansi) DENGAN METODE ABTS. Other thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

The use of kluwih plants so far has only been focused on the fruit and stems, while the leaves of kluwih (Artocarpus camansi) also contain many active compounds, especially flavonoids. The purpose of this study was to determine the total flavonoid levels and antioxidant activity of the extract and fraction of kluwih leaves (Artocarpus camansi). The extract was made by maceration method using 70% ethanol solvent followed by the addition of warm water then fractionation using n-hexane and ethyl acetate solvents. Determination of flavonoid levels and antioxidant activity was carried out using a UV-Vis spectrophotometer. The antioxidant activity was based on the free radical scavenging ability of 2,2'-azinobis (3-ethylbenzthiazoline 6- sulfonic acid) (ABTS) compounds measured at a wavelength of 427.5 nm at 2 minutes. The analysis was conducted to determine the differences in total flavonoid levels and antioxidant activity between extracts and fraction of kluwih leaves (Artocarpus camansi) using One Way ANOVA. In phytochemical screening, extracts and fractions of kluwih leaves contain flavonoids. The results of the determination of the total flavonoid mean content in the ethanol extract were 2.9950 ± 0.0196% QE, the n-hexane fraction was 1.0262 ± 0.0175% QE, the ethyl acetate fraction was 2.5019 ± 0.0468% QE , and the water fraction of 1.5165 ± 0.0270% QE. The average antioxidant activity is expressed in IC50 values with the results in the ethanol extract of 16.7181 ± 0.2318 ppm, the nhexane fraction 23.0089 ± 0.3990 ppm, the ethyl acetate fraction 18.2579 ± 0.2936 ppm , and the water fraction of 18.5776 ± 0.0697 ppm. Based on one way ANOVA analysis on total flavonoid levels, there was a significant difference between ethanol extract and kluwih leaf fraction (Artocarpus camansi) (p <0.05). There was a significant difference in antioxidant activity between ethanol extract and kluwih leaf fraction (Artocarpus camansi) (p <0.05).

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