PENETAPAN KADAR KUERSETIN PADA PERASAN BAWANG BOMBAY (Allium cepa L) SECARA HPLC (HIGH PERFORMANCE LIQUID CHROMATOGRAPHY)

Maharani, Pitaningtyas (2019) PENETAPAN KADAR KUERSETIN PADA PERASAN BAWANG BOMBAY (Allium cepa L) SECARA HPLC (HIGH PERFORMANCE LIQUID CHROMATOGRAPHY). Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Quercetin an inhibitor of α-amylase enzyme that functions in the breakdown of carbohydrates, thus solving process and absorption of carbohydrates will interfere so that the blood glucose level in hyperglycemia can be derived. This study aims to determine the levels of quercetin in the juice of bulb onion (Allium cepa L). The analytical method used is the reversed phase HPLC (High Performance Liquid Chromatography) with C18 column (Oktadesilsilika) as stationary phase and methanol: 0.1% ortho phosphotic acid (65: 35) as mobile phase, which flow rate of 1 ml / min. The detector is set at a wavelength of 371 nm and the maximum injection volume is set at 20 μL. Standard curve created in the concentration range of 2 to 10 ppm obtained linearity with correlation coefficient 0.9986. The results showed that in a sample of juice bulb onions (Allium cepa L) contain quercetin positive with levels of 0.7087 mg% and the coefficient of variation of 1.4014%.

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