PENETAPAN KADAR KUERSETIN EKSTRAK METANOL BAWANG BOMBAY (Allium cepa L.) SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Muhamad, Hidayat (2019) PENETAPAN KADAR KUERSETIN EKSTRAK METANOL BAWANG BOMBAY (Allium cepa L.) SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC). Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Bombay Onion (Allium cepa L.) is part plant bulbs are often consumed the community for seasoning and has a content of quercetin can decrease the levels of sugar in the blood. Fresh Bombay Onion macerated using methanol by comparison (1:8) phase I and comparison (1:4) phase II with a yield of 13,48%. This research was conducted to find out the levels of quercetin contained in methanol extracts of Bombay onion by reversed phase High Performance Liquid Chromatography (HPLC). The stationary phase used is oktadesil silica (C18) with mobile phase methanol : 0,1 % phosphoric acid (65:35), which is conducted with a flow rate of 1 ml/min. HPLC detector set at 371 nm wavelength. The quercetin standard solution is made on the range of concentration of 2-10 ppm with correlation coefficient (r) produced 0,9957. The result of quercetin methanol extract determination in Bombay onion is 0,4698% b /b with coefficient of variation is 2,3929%

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