UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BUNGA PEPAYA (Carica papaya L.) DENGAN METODE ABTS (2,2 azinobis (3-etilbenzotiazolin)-6-asam sulfonat)

Tika, Ayu Lusiyaningrum (2021) UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BUNGA PEPAYA (Carica papaya L.) DENGAN METODE ABTS (2,2 azinobis (3-etilbenzotiazolin)-6-asam sulfonat). Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Papaya flower are known to contain high flavonoid compounds, in which one of the effects of flavonoids is as antioxidant. This study aims to determine antioxidant activity of papaya flower by ABTS method (2,2- Azinobis(ethylbenzothiazoline)-6-sulfonic acid), and tested its phytochemicals. Papaya flower are made into a thick extract using maceration. The antioxidant activity was measured by ABTS method with visible spectrophotometer at maximum wavelength of 734 nm to obtain IC50 using vitamin C standard as positive control. The presence of flavonoid compound was identified with magnesium powder and concentrated HCl as reagen. The results showed that papaya flower had antioxidant activity with IC50 value of 13,2755 ppm, whereas vitamin C standard had IC50 value of 4,8946 ppm. Papaya flower ethanol extract and vitamin C are very powerful antioxidants.

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