UJI DAYA HAMBAT EKSTRAK ETANOL 96% DAUN PETAI (Parkia speciosa) TERHADAP BAKTERI Pseudomonas aeruginosa

Annissa, Annissa (2021) UJI DAYA HAMBAT EKSTRAK ETANOL 96% DAUN PETAI (Parkia speciosa) TERHADAP BAKTERI Pseudomonas aeruginosa. Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Pseudomonas aeruginosa is a bacterium that is able to adapt to low oxygen and nutrient conditions. Pseudomonas aeruginosa can also infect the lungs, so these bacteria can also be co-infected with the Covid-19 virus. In petai plants (Parkia speciosa) contains phenolic compounds. In this study using extract concentrations of 25%, 50%, 75%, and 100% which will then be used for antibacterial testing. The purpose of this determination was to determine the ability of 96% ethanol extract of petai leaves (Parkia speciosa) in inhibiting the growth of Pseudomonas aeruginosa bacteria and to determine the concentration of 100% ethanol extract of petai leaves (Parkia speciosa) which produced the greatest inhibition zone on the growth of Pseudomonas aeruginosa bacteria. This research uses experimental research type. The method used is Kirby & bauer which uses a blank disk dipped in the extract and Nutrient Agar media. The results obtained from this study from a positive control using the antibiotic Ciprofloxacin from 4 replications obtained an average of 28.9 mm, and for 4 variants the concentration did not show any inhibition zones. The conclusion is that 96% ethanol extract of petai leaves (Parkia spciosa) is not able to inhibit the growth of Pseudomonas aeruginosa bacteria. At a concentration of 100% ethanol extract 96% petai leaves (Parkia spciosa) could not produce an inhibition zone as well as in the concentration variants of 25%, 50%, and 75%.

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