AKTIVITAS ANTIOKSIDAN FRAKSI DAUN PEGAGAN (Centella asiatica (L) urban) DENGAN METODE FRAP

Aristika, Linda Puspita (2021) AKTIVITAS ANTIOKSIDAN FRAKSI DAUN PEGAGAN (Centella asiatica (L) urban) DENGAN METODE FRAP. Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Gotu kola is a plant that contains vitamin C, vitamin C is a very powerful antioxidant. The purpose of this study was to determine the highest antioxidant potential in three fractions of gotu kola extract. Qualitative test using phosphate and ferricyanide buffer reagent gave yellow color, and FeCl reagent gave blue to blue berlin. The quantitative test was performed using UV-Vis spectrophotometry with a wavelength of 676.0 nm and operating time 27 minutes. The results of the quantitative test showed that the antioxidant capacity of each fraction were ethyl acetate, water phase and N hexane. The average antioxidant capacity of ethyl acetate was 0.09349, water phase 0.08742, Nhexane 0.0621. In the ANOVA test, the results showed p = 0.714 which means that it is greater than 0.05 that there is no significant difference or no significant difference in vitamin C levels.

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