PENETAPAN KADAR KUERSETIN PADA SARI PERASAN BUAH BUNCIS (Phaseolus vulgaris L.) SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Laras, Setya Maharani (2019) PENETAPAN KADAR KUERSETIN PADA SARI PERASAN BUAH BUNCIS (Phaseolus vulgaris L.) SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

String beans (Phaseolus vulgaris L) is one of the types of plants that are widely used and easily found in various places. String beans contain compounds quercetin has proven efficacy in lowering glucose levels in the blood. The purpose of this research is to determine the levels of quercetin in the juice of string beans by reverse phase High Performance Liquid Chromatography (HPLC), the stationary phase is C18 column (Octadecyl xylane), mobile phase is methanol : 0,1 % phosphoric acid (65:35) with flow rate 1,0 mL/minute. The detector is set to a UV 371,0 nm. The results showed that the juice of string beans positive indication contains quercetin at the range retention time from 6,831 to 7,222 minutes. Levels Quercetin in the sample that is 0,0341 mg% in the juice. This study qualifies the test of precision and linearity, because in the range quercetin standart 0,2 – 1,0 ppm produces coefficient of variation of the sample is 4,707% and coefficient correlation is 0,9976.

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