RESISTENSI BAKTERI GRAM NEGATIF TERHADAP ANTIBIOTIK CEFOTAXIME DARI SAMPEL KULTUR LABORATORIUM BAKTERIOLOGI STIKES NASIONAL

Luluk, Damayanti (2021) RESISTENSI BAKTERI GRAM NEGATIF TERHADAP ANTIBIOTIK CEFOTAXIME DARI SAMPEL KULTUR LABORATORIUM BAKTERIOLOGI STIKES NASIONAL. Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Bacterial culture is a method of multiplying bacteria on culture media by culturing in an aseptically controlled laboratory, to determine the type of bacteria. However, in the STIKES Nasional bacteriology laboratory, there are 8 specises of gram-negative bacterial culture samples such as Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Serratia marcescens, Salmonella typhosae, Proteus mirabilis, Providencia rettgeri and Pseudomonas aeruginosa. Where the bacterial culture is used periodically as a sample for bacterial examination in the bacteriological laboratory practicum at STIKES Nasional where the sampel has never been monitored for resistance to the antibiotic Cefotaxime. The purpose of this study was to determine the gram-negative bacteria Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Serratia marcescens, Salmonella typhosae, Proteus mirabilis, Providencia rettgeri and Pseudomonas aeruginosa bacteriology laboratory culture samples from National STIKES that are resistant to Cefotaxime antibiotics. This study uses a descriptive design. This research was conducted at the Bacteriology Laboratory of the National College of Health Sciences and the time of this research was carried out on March 22-16 April 2021. The samples of this study were 8 species of gram-negative bacteria using the antibiotic Cefotaxime 30μg. Disc diffusion method (Kirby Bouer test) and the sampling technique used is purposive sampling. The results of this study showed that the bacteria Klebsiella pneumoniae, Klebsiella oxytoca and Pseudomonas aeruginosa were resistant to the antibiotic Cefotaxime 30 g with the resulting radical zones of 6 mm, 22 mm and 19 mm, respectively. For bacteria Serratia marcescens, Salmonella typhosae, Proteus mirabilis and Providencia rettgeri were still sensitive to the antibiotic Cefotaxime 30 g with the results of radical zones 31 mm, 33 mm, 28 mm and 54 mm, respectively. While Escherichia coli bacteria intermediate to Cefotaxime 30 g radical zone formed by 24 mm. Bacteria Klebsiella pneumoniae, Klebsiella oxytoca and Pseudomonas aeruginosa aeruginosa were resistant to the antibiotic Cefotaxime 30 g. The bacteria Serratia marcescens, Salmonella typhosae, Proteus mirabilis and Providencia rettgeri were still sensitive to the antibiotic Cefotaxime 30 antibiotikg and the intermediate Escherichia coli bacteria to Cefotaxime 30 g.

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