GAMBARAN PEMBENTUKAN PIGMEN Serratia marcescens PADA BEBERAPA MEDIA ISOLASI DENGAN VARIASI WAKTU INKUBASI

Muhammad, Khaidir Munazi (2021) GAMBARAN PEMBENTUKAN PIGMEN Serratia marcescens PADA BEBERAPA MEDIA ISOLASI DENGAN VARIASI WAKTU INKUBASI. Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Serratia marcescens is a gram-negative bacterium that has a thin cell wall made of a single layer of peptidoglycan covered by an outer membrane. Serratia marcescens bacteria is one of the red pigment-producing bacteria that is widely used as a natural dye and has a characteristic in its growth medium that can distinguish it from other bacteria, namely the red pigment prodigiosin. Factors that influence the formation of pigments are the source of carbon, nitrogen of each insulating medium, temperature and length of incubation time. The purpose of this study was to describe the formation of Serratia marcescens pigment in several isolation media with variations in incubation time. The study used a descriptive design. This research was conducted at the National STIKES Bacteriology Laboratory and the time of the study was conducted in January-May. The sample of this study was the bacterial culture of Serratia marcescens, NA, MHA, TSA, BHI and NA slanted isolation media with variations in incubation time of 20, 24, 48, 72 and 75 hours. The sampling technique used is Total Sampling. The results showed that the formation of Serratia marcescens pigment production on Nutrient Agar (NA) media only 20 hours incubation time. Meanwhile, the isolation media of MHA, TSA, BHI and NA were skewed for the formation of Serratia marcescens pigment with an incubation time of 20 hours to 75 hours did not show the formation of pigment production. It can be concluded that each isolation medium used in this study Pigment Serratia marcescens only formed on Nutrient Agar (NA) media at an incubation time of 20 hours the color began to fade after incubation 24 hours to 75 hours and for MHA, TSA, BHI, NA media it was not skewed. showed a different appearance when incubated with variations in incubation time.

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