UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL, FRAKSI nHEKSANA, ETIL ASETAT, DAN AIR DAUN BIT (Beta vulgaris L.) TERHADAP BAKTERI Escherichia coli ESBL

Dewi, Anjaswati (2021) UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL, FRAKSI nHEKSANA, ETIL ASETAT, DAN AIR DAUN BIT (Beta vulgaris L.) TERHADAP BAKTERI Escherichia coli ESBL. Other thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Inappropriate use of antibiotics can lead to resistance problems. One contribution to the occurrence of resistance is the presence of bacteria that produce ESBL (Extended Spectrum Beta lactamase). Based on this, it is necessary to find alternative medicine by utilizing active compounds in plants, namely beet leaves. Beet leaves were extracted using maceration method with 96% ethanol. The extract was then fractionated using n-hexane, ethyl acetate, and water as solvent. The resulting extracts and fractions were then tested for phytochemical and antibacterial tests using the disc diffusion method with concentrations of 25%, 50%, and 100% against Escherichia coli ESBL bacteria. The diameter of the inhibition zone obtained was then analyzed statistically. Beet leaf extract and ethyl acetate fraction had antibacterial activity at each concentration. The water fraction has activity at concentrations of 50%, and 100%. Water fraction at concentration 25% did not show any antibacterial activity. The n-hexane fraction did not show any antibacterial activity at any concentration. The results of the comparison of the antibacterial activity of the extract and the beet leaf fraction with a statistically positive control using the Mann Whitney test showed an asymp sig value <0.05, which means that there was a significant difference in antibacterial activity between the extract and the beet leaf fraction against the positive control. However, the 100% ethyl acetate fraction had the same inhibition as chloramphenicol which was 20.58 mm (≥ 18 mm) based on the CLSI 2020.

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