PERBEDAAN KADAR HEMOGLOBIN MENGGUNAKAN ANTIKOAGULAN K2EDTA YANG DIPERIKSA SEGERA DAN DITUNDA 2 JAM

Nadea, Novita (2021) PERBEDAAN KADAR HEMOGLOBIN MENGGUNAKAN ANTIKOAGULAN K2EDTA YANG DIPERIKSA SEGERA DAN DITUNDA 2 JAM. Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Blood sampling for hemoglobin level examination should be checked immediately after blood collection. There are EDTA anticoagulants that are often used, namely K2EDTA and K3EDTA. Examination using EDTA blood should be carried out immediately, if it has to be postponed, it must pay attention to the examination time limit. In this study, the anticoagulant K2EDTA was used which was recommended by ICSH because it has stability in maintaining cell shape and size. The purpose of this study was to determine the difference in hemoglobin levels that were checked immediately and delayed by 2 hours. This type of research is experimental analytic using a cross sectional approach. The sampling technique in this study is total sampling. The subjects of this study were students of class 3A2 of the DIII Medical Laboratory Technology Study Program at the Surakarta National Health Sciences College which opened 37 people. This research was conducted in the Laboratory of Hematology, National Health Sciences College, Surakarta. Data were obtained through filling out informed consent. Hemoglobin levels were calculated using the cyanmethemoglobin method. Normality test was performed using the Shapiro-Wilk test and was not normally distributed. The data was processed using a non-parametric test, namely the Wilcoxon test and the p value (0.00) < (0.05). The conclusion of this study is that there is a difference in hemoglobin levels using the anticoagulant K2EDTA which is checked immediately and delayed by 2 hours.

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