PENGARUH VARIASI KONSENTRASI BENGKUANG (Pachyrrhizus erosus) SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN Staphylococcus aureus

Alvindo, Thiananda Devi (2022) PENGARUH VARIASI KONSENTRASI BENGKUANG (Pachyrrhizus erosus) SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN Staphylococcus aureus. Other thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

The increase in examinations in microbiology laboratories makes the use of nutrient agar (NA) general media increased, this media also has a relatively expensive price. Yam bean (Pachyrrhizus erosus) is a natural ingredient in making alternative media that contains various nutrients for the growth and development of bacteria, and has a relatively cheap price, and is easy to obtain. This study aims to determine the influence of variations in P. erosus concentrations as an alternative medium for the growth of Staphylococcus aureus, as well as the characteristics of S. aureus colonies on alternative media P. erosus. This study was experimental with the posttest only with control group research design using six replications in NA positive control media, negative control media and alternative media P. erosus concentrations of 10%, 15%, 20%. Based on the results of the study, there were differences in size characteristics and the appearance of S. aureus colony pigments in alternative media P. erosus with NA media. Colonies of S. aureus in alternative media P. erosus have a smaller size with the appearance of pigments that are slower than NA media, as well as the average growth of S. aureus colonies in each concentration of alternative media P. erosus 10%, 15%, 20% i.e. 3 × 106 CFU/mL, 7.5 × 106 CFU/mL, 123.8 × 106 CFU/mL. The results of the Analysis of One Way ANOVA test data were obtained by Sig results. < 0.005 which means there is an influence of variation in yam bean concentration (P. erosus) as an alternative medium of S. aureus growth. The results of the LSD Post-Hoc Test showed that alternative media P. erosus concentrations of 20% sig (0.000) < 0.005 had significant differences in all concentrations (10%, 15%) and positive control of NA.

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