PEMANFAATAN KOMBINASI SARI UBI JALAR UNGU DAN BUAH BIT SEBAGAI ALTERNATIF PEWARNAAN GRAM

Indra, Ayu Vidyaningrum (2022) PEMANFAATAN KOMBINASI SARI UBI JALAR UNGU DAN BUAH BIT SEBAGAI ALTERNATIF PEWARNAAN GRAM. Other thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Synthetic dyes in gram stain aim to sharpen and clarify the picture of cells in bacteria so that they are easier to observe under a microscope. Synthetic dyes have the disadvantage of being expensive and easily damaged. Therefore, alternative dyes are needed as a substitute or as another option for synthetic dyes. The purpose of this study was to determine whether the combination of purple sweet potato juice and beetroot was able to color cells. The research method was descriptive experimental. The population of this research is purple sweet potato and beetroot which are obtained in the Sragen market. Sampling technique by means of quota sampling. Purple sweet potato and beetroot were washed and peeled, then grated and weighed as much as 100g then squeezed with filter paper, purple sweet potato juice and beetroot were stored. Both were homogenized 10ml each. Bacterial samples from ATCC 29213 (E.coli) and ATCC 25922 (S.aureus) which had been prepared were stained with a combination of purple sweet potato juice and beetroot. This study used a quasi-experimental design where pretest posttest was used in the control group and the experimental group. The results showed that the combination of purple sweet potato juice and beetroot was not able to differentiate between gram-positive bacteria and gram-negative bacteria but was able to stain bacterial cells. The conclusion that the combination of purple sweet potato juice and beetroot cannot be used as a substitute for the main dye in Gram staining

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