PEMANFAATAN UBI JALAR PUTIH DAN UBI JALAR KUNING SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN Staphylococcus aureus

Sarita, Diah Kusuma Arum (2022) PEMANFAATAN UBI JALAR PUTIH DAN UBI JALAR KUNING SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN Staphylococcus aureus. Other thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Staphylococcus aureus is one of the pathogens that cause severe infection in humans. Staphylococcus aureus culture is commonly using Nutrient Agar medium, the researcher needs to find alternative mediums from nature such as white-fleshed sweet potato and yellow-fleshed sweet potato. This research aims to find the difference in Staphylococcus aureus growth ability in white-fleshed sweet potato medium, yellow-fleshed sweet potato medium, and Nutrient Agar. This type of research is an experimental post-test-only control group design using spread plate method. that. Staphylococcus aureus suspense was inoculated into each growth medium and then incubated at 37°C for 72 hours. The determination of characteristics and number of colonies of Staphylococcus aureus are made every 24 hours of incubation. The number of colonies of Staphylococcus aureus at 48 hours of incubation was 15,8 x 10^6 CFU/ml on NA medium, 125 x 10^6 CFU/ml on white-fleshed sweet potato medium, and 17,1 x 10^6 CFU/ml on yellow-fleshed sweet potato medium. The One-Way ANNOVA test is assumed that there is a significant difference (p<0,005). The post-hoc test is used as an advanced test. The results showed that there are differences in the ability of the growth media to grow Staphylococcus aureus. Yellow-fleshed sweet potato media and NA medium had the same ability, but white-fleshed sweet potato media was better at growing Staphylococcus aureus than yellow-fleshed sweet potato media.

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