PERBEDAAN CAIRAN FIKSASI TERHADAP KUALITAS GAMBARAN MIKROSKOPIS PADA JARINGAN DENGAN PERWARNAAN HEMATOXYLIN-EOSIN (HE)

Defi, Pramitaningrum (2020) PERBEDAAN CAIRAN FIKSASI TERHADAP KUALITAS GAMBARAN MIKROSKOPIS PADA JARINGAN DENGAN PERWARNAAN HEMATOXYLIN-EOSIN (HE). Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Fixation is one of the histotechnical stages that aims to inhibit the process of decay, autolysis, preserving or maintaining the shape of cells that exist in the tissue to remain intact, colloidal solidification and effect on staining. The purpose of this study was to determine fixation fluids with better penetration power to the quality of microscopic images in tissues after the staining of Hematoxylin-Eosin (HE). This study uses a type of literature study by collecting reference data obtained from research results relating to differences in fluid fixation in tissues with Hematoxylin-Eosin (HE) staining such as fixative fluids Neutral Buffer Formalin (NBF) 10%, Alcohol 70%, Alcohol, Bouin and Methanol with research sample is liver tissue. The data obtained were then analyzed using descriptive analysis. The results obtained, that the quality of a good tissue preparation for fixation is the type of 10% NBF solution and Bouin which results show the quality of the preparation was observed and an assessment of score 3 with good results that appear blue in the cell nucleus, red color in the cytoplasm is clear and the colors in the preparations are uniform, whereas in tissues fixed with 70% Alcohol fixation fluid, Acetone and Methanol with a score of score 2 with unfavorable results that show the results of blue in the cell nucleus less, red in the cytoplasm as well as colors in the preparations less uniform.

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