ANALISIS RESIDU PESTISIDA KLORPIRIFOS PADA DAUN BAWANG (Allium fistulosum L.) SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Septi, Rama Anisa (2020) ANALISIS RESIDU PESTISIDA KLORPIRIFOS PADA DAUN BAWANG (Allium fistulosum L.) SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC). Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Chlorpyrifos is an organophosphate group pesticide that is widely used by farmers. The maximum limit of pesticide residues is the concentration that is permissible in agricultural crops. Welsh onion (Allium fistulosum L.) is one of the plants that is exposed to pesticides, so research is conducted to determine the residual levels of chlorpyrifos pesticides contained in welsh onion (Allium fistulosum L.), and to determine compliance with the maximum limit of pesticide residues has been set at 0.05 mg/kg. The method used in this study uses High Performance Liquid Chromatography with a stationary phase C18 column (Octadecyl silica), a water phase: acetonitrile (50:50) and a flow rate of 0.70 ml/min. Analysis of chlorpyrifos pesticide residues was carried out using dichloromethane solvents, with the detector set at a wavelength of 291 nm. The results of the study showed the average levels of chlorpyrifos residues contained in welsh onion were 131.6023 mg/kg and the coefficient of variation was 2.0388%, these results did not meet the maximum limit of pesticide residues (BMR).

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