PENETAPAN KADAR FLAVONOID TOTAL PERASAN DAUN KEMANGI (Ocimum sanctum L) YANG BERPOTENSI SEBAGAI HEPATOPROTEKTOR DENGAN METODE SPEKTROFOTOMETRI UV-VIS

Berlina, Intan Yuniar (2020) PENETAPAN KADAR FLAVONOID TOTAL PERASAN DAUN KEMANGI (Ocimum sanctum L) YANG BERPOTENSI SEBAGAI HEPATOPROTEKTOR DENGAN METODE SPEKTROFOTOMETRI UV-VIS. Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Potential plants as hepatoprotectors are caused by antioxidant activity that can prevent lipid peroxidation that damages liver cells. Kemangi leaves contain flavonoid compounds that can be used as antioxidants. The aim of this research was to determine the total flavonoid levels in the juice of kemangi (Ocimum sanctum L). Extract that can be used for qualitative and quantitative analysis, quercetin is chosen as the standard. Qualitative analysis was done with concentrated HCL reagents and magnesium metal causing a change in color to red which indicates a positive sample containing flavonoids and the addition of NaOH reagents giving rise to a positive yellow color. Quantitative analysis was performed by UV-Vis spectrophotometry using a wavelength of 432 nm and operating Time at the 24th minute. The results of quantitative analysis showed total flavonoid levels contained in the juice of kemangi leaves with an average flavonoid level of 0.001922 % (w / w) and variation coefficient of 0.3855%.

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