UJI OBSERVASI PEMBENTUKAN HASIL FERMENTASI OLEH BAKTERI GRAM NEGATIF PADA MEDIA TSIA PADA BERBAGAI VARIASI WAKTU INKUBASI

Mutiara, Diva Salsabila (2021) UJI OBSERVASI PEMBENTUKAN HASIL FERMENTASI OLEH BAKTERI GRAM NEGATIF PADA MEDIA TSIA PADA BERBAGAI VARIASI WAKTU INKUBASI. Diploma thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

TSIA is a differential medium with a pH indicator that can distinguish microorganisms based on their ability to decompose specific carbohydrates, namely glucose and lactose or sucrose with or without producing gas. The media used has two parts, namely slant and butt. Changes experienced after incubation, namely the color of the medium yellow indicates acid, the color of the medium becomes redder indicates the medium becomes alkaline. The aims of this study was to determine the reading time of the results of the formation of fermentation on TSIA media by several gram-negative bacteria at various incubation times. This study used a descriptive observational design. This study was conducted at the STIKES Nasional Bacteriology Laboratory and the time of the study was carried out in January-May. The samples of this study were bacterial cultures of Escherichia coli, Klebsiella pneumoniae, Serratia marcescene, and Providencia rettgeri, isolated media of TSIA (Triple Sugar Iron Agar) which were incubated with variations in incubation time of 8, 16, 24, and 32 hours. The sampling technique used is Quota Sampling. The results of study showed that results of acid/acid in Escherichia coli and Klebsiella pneumoniae bacteria within an incubation time of 8 hours fermenting 4 /4 parts slant and butt. For alkaline/acid results in Serratia marcescene, the acidic part of the butt was fermented 4 /4 parts within an incubation time of 8 hours, while for Providencia rettgeri bacteria the acidic part of the butt was fermented 3 /4 parts at an incubation time of 8 hours. Based on the results of study that has been carried out regarding the observation test for the formation of fermented products on TSIA media at various incubation times, it can be concluded that there are differences in the results of acid/acid and alkaline/acid fermentation on TSIA media produced by bacteria Escherichia coli, Klebsiella pneumoniae, Serratia marcescene and Providencia rettgeri at various incubation times of 8, 16, 24, and 32 hours.

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