UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI n-HEKSAN, ETIL ASETAT DAN AIR DARI DAUN SALAM (Syzygium polyanthum [Wight.] Walp) TERHADAP BAKTERI Salmonella typhi MENGGUNAKAN METODE DIFUSI CAKRAM

Mahanani, Eka Barata (2021) UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI n-HEKSAN, ETIL ASETAT DAN AIR DARI DAUN SALAM (Syzygium polyanthum [Wight.] Walp) TERHADAP BAKTERI Salmonella typhi MENGGUNAKAN METODE DIFUSI CAKRAM. Other thesis, Sekolah Tinggi Ilmu Kesehatan Nasional.

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Abstract

Bay leaves contain alkaloids, flavonoids, saponins and tannins which have antibacterial activity. This study aims to determine the antibacterial potential of bay leaf extract and fraction against the growth of Salmonella typhi bacteria and compare the antibacterial activity of bay leaf extract and fraction against Ciprofloxacin positive control. Bay leaves were macerated with 70% ethanol then dissolved in warm water and fractionated using n-hexane and ethyl acetate as solvents. The results of extraction and fractionation were carried out at concentrations of 25%, 50%, 75% and 100% on the antibacterial activity test against Salmonella typhi using the disk diffusion method. The positive control used Ciprofloxacin while the negative control used 10% DMSO. The results of the diameter of the radical inhibition zone were analyzed using Kruskal Wallis. The results showed that the ethanol extract and bay leaf fraction were able to inhibit Salmonella typhi bacteria. The largest radical inhibition zone was in the ethyl acetate fraction with a concentration of 75%, which was 21.57 mm, including the sensitive category but the value was significantly different (p≤ 0.05) with the positive control Ciprofloxacin 42.08 mm. The results of the comparison of antibacterial activity of bay leaf extract and fraction with positive control statistically using the Mann Whitney test showed a value of asymp sig 0.05, which means there is a significant difference.

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